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A novel Coronavirus, currently termed 2019-nCoV, was announced as the etiological agent of cases of ongoing Pneumonia outbreak in Wuhan City. This kit describes the use of real time RT-PCR for the in vitro detection of 2019-nCoV in respiratory specimens (sputum; nasopharyngeal, oropharyngeal aspirates, washes or swabs; tracheal aspirates).
qMAXSen™ Coronavirus (SARS-CoV-2) RT-qPCR Detection Kit allows efficient cDNA synthesis and Real-Time PCR in a single tube. The kit includes a qPCR MasterMix supplied in a 2x concentration to perform real-time PCR. qPCR MasterMix (2x) contains all the reagent (except PCR primers, probe and template) needed for running PCR reactions. In addition, a separate RT Mix that comprises a balanced mixture of both RTase (Reverse Transcriptase) and RNase Inhibitor is also provided.
Additionally, the kit contain one sets of Primers and Fluorescent Probes to differentiate between 2019-nCoV and SARS-CoV (or bat SARS related CoV). The probes are readout in different channels. Coronavirus 2019-nCoV RNA targets are amplified and detected in the FAM channel, Coronavirus SARS-CoV (or bat SARS related CoV) RNA targets are amplified and detected in the HEX, VIC or JOE channel (depending on the equipment used).
Also, it includes a Positive Control (PTC) and Negative Control (NTC). The positive control is supplied to demonstrate that the PCR amplification is working efficiently with the supplied components. To confirm absence of contamination, a negative control reaction should be included every time the kit is used.